Lynd Research Lab: Metabolic Engineering

The Lynd Lab's Metabolic Engineering group specializes in development and application of techniques for the genetic manipulation of cellulose- and hemicellulose-utilizing anaerobic bacteria pursuant to improving their properties and performance for production of cellulosic biofuels.

Ten most recent papers:

  • Biswas, R., T. Zheng, D.G. Olson, L.R. Lynd, A.M. Guss. 2015. Elimination of hydrogenase active site assembly blocks H2 production and increases ethanol yield in Clostridium thermocellum. Biotechnol. Biofuels. 8:20. (Abstract)
  • Bhandiwad, A., A.J. Shaw, A. Guss, A. Guseva, H. Bahl, L.R. Lynd. 2014. Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production. Metab. Eng. 21:17-25. (Article)
  • Brown, S.D., D.M. Klingeman, C.M. Johnson, A. Clum, A. Aerts, A. Salamov, A. Sharma, M. Zane, K. Barry, I.V. Grigoriev, B.H. Davison, L.R. Lynd, P. Gilna, H. Hau, D.A. Hogsett, A.C. Froehlich. 2013. Genome Sequences of Industrially Relevant Saccharomyces cerevisiae Strain M3707, Isolated from a Sample of Distillers Yeast and Four Haploid Derivatives. Genome Announc. 1(3):e00323-13. (Abstract)
  • van der Veen, D., J. Lo, S.D. Brown, C.M. Johnson, T.J. Tschaplinski, M. Martin, N.L. Engle, R.A. van den Berg, A.D. Argyros, N.C. Caiazza, A.M. Guss, L.R. Lynd. 2013. Characterization of Clostridium thermocellum strains with disrupted fermentation end-product pathways. J. Ind. Microbiol. Biotechnol. 40(7):725-734. (Abstract)
  • Deng, Y., D.G. Olson, J. Zhou, C.D. Herring, A.J. Shaw, L.R. Lynd. 2013. Redirecting carbon flux through exogenous pyruvate kinase to achieve high ethanol yields in Clostridium thermocellum. Metab. Eng. 15:151-158. (Article)
  • Bhandiwad, A., A. Guseva, L. Lynd. 2013. Metabolic engineering of Thermoanaerobacterium thermosaccharolyticum for increased n-butanol production. Adv. Microbiol. 3:46-51. (Abstract)
  • Currie, D.H., C.D. Herring, A.M. Guss, D. Olson, D.A. Hogsett, L.R. Lynd. 2013. Functional heterologous expression of an engineered full length CipA from Clostridium thermocellum in Thermoanaerobacterium saccharolyticum. Biotechnol. Biofuels. 6:32. (Article)
  • Olson, D.G., L.R. Lynd. 2012. Transformation of Clostridium thermocellum by electroporation. Method Enzymol. 510:317-330. (Abstract)
  • Mearls, E.B., J.A. Izquierdo and L.R. Lynd. 2012. Formation and characterization of non-growth states in Clostridium thermocellum: spores and L-forms. BMC Microbiol. 12:180. (Abstract)
  • Olson, D.G. and L.R. Lynd. 2012. Computational design and characterization of a temperature sensitive plasmid replicon for gram positive thermophiles. J. Biol. Eng. 6:5. (Abstract)

Selected additional papers:

  • Guss, A.M., D.G. Olson, N.C. Caiazza and L.R. Lynd. 2012. Dcm methylation is detrimental to plasmid transformation in Clostridium thermocellum. Biotechnol. Biofuels. 5:30. (Abstract)
  • Argyros, D.A., S.A. Tripathi, T.F. Barrett, S.R. Rogers, L.F. Feinberg, D.G. Olson, J.M. Foden, B.B. Miller, L.R. Lynd, D.A. Hogsett and N.C. Caiazza. 2011. High ethanol titers from cellulose by using metabolically engineered thermophilic, anaerobic microbes. Appl. Environ. Microbiol. 77(23):8288-8294. (Article)
  • Olson, D.G., J.E. McBride, A.J. Shaw and L.R. Lynd. 2011. Recent progress in consolidated bioprocessing. Curr. Opin. Biotech. 23(3):396-405. (Article)
  • Brown, S.D., A.M. Guss, T.V. Karpinets, J.M. Parks, N. Smolin, S. Yang, M.L. Land, D.M. Klingeman, A. Bhandiwad, M. Rodriguez, B. Raman, X. Shao, J.R. Mielenz, J.C. Smith, M. Keller and L.R. Lynd. 2011. Mutant alcohol dehydrogenase leads to improved ethanol tolerance in Clostridium thermocellum. PNAS. 108(33):13752-13757. (Abstract)
  • Shao, X., B. Raman, M. Zhu, J.R. Mielenz, S.D. Brown, A.M. Guss, and L. R. Lynd. 2011. Mutant selection and phenotypic and genetic characterization of ethanol-tolerant strains of Clostridium thermocellum. Appl. Microbiol. Biot. 92(3):641-652. (Abstract)
  • Tripathi, S.A., D.G. Olson, D.A. Argyros, B.B. Miller, T.F. Barrett, D.M. Murphy, J.D. McCool, A.K. Warner, V.B. Rajgarhia, L.R. Lynd, D.A. Hogsett and N.C. Caiazza. 2010. Development of pyrF-based genetic system for targeted gene deletion in Clostridium thermocellum and creation of a pta mutant. Appl. Environ. Microbiol. 76(19):6591-6599. (Abstract)
  • Shaw, A.J., D.A. Hogsett, L.R. Lynd. 2010. Natural competence in Thermoanaerobacter and Thermoanaerobacterium species. Appl. Environ. Microbiol. 76:4713-4719. (Abstract)
  • Shaw, A.J., D.A. Hogsett, L.R. Lynd. 2009. Identification of the [FeFe]-hydrogenase responsible for hydrogen generation in Thermoanaerobacterium saccharolyticum and demonstration of increased ethanol yield via hydrogenase knockout. J. Bacteriol. 191:6457-6464. (Abstract)
  • Shaw, A.J., K. Podkaminer, S.G. Desai, J.S. Bardsley, S.R. Rogers, P.G. Thorne, D.A. Hogsett, L.R. Lynd. 2008. Metabolic engineering of a thermophilic bacterium to produce ethanol at high yield. PNAS. 105:13769-13774. (Abstract)